Study of some growth and hematology indices of catfish Pangasianodon hypophthalmus after diet supplementation with Salvia macrosiphon extract

The improvement of immune system and increasing growth and survival of fishes especially at early stages is important for fish culturists. Considering the effects of herbal immunostimulants on innate immunity and growth of fish, the impacts of dietary Salvia macrosiphon extract on some growth and immunological parameters of fingerlings Pangasianodon hypophthalmus were investigated. Fishwith mean weight of 1.27±0.24 were fed diets supplemented with S. macrosiphon extract at 0 (control group), 150, 300 and 600 mg/kg diet for 45 days. After the feeding trial, fish growth and hematological parameters was analyzed using Duncan test at 95% confidence. Fish growth parameters were not significantly different between treatments at days 15, 30 and 45. All the treatments showed almost 100% of survival. The lowest haematocrit level was observed at control (41.33 ± 4.16%), while the highest (44.56± 2.00%) was at 300 mg/kg diet of Salvia extract. Mean number of WBC and RBC was not different between the treatments. WBC differential count revealed that the highest percentage of neutrophil was in treatment 2, the highest percentage of lymphosyte in treatment 1 and the lowest percentage of monocyte in treatment 3. The mean values of MCV, MCH, MCHC, Hemoglobin, Albumin and total protein had no significant difference between the treatments. The amount of lysozyme was significantly different with the highest amount in treatments 2 and the lowest in treatment 3 (p<0.05).